Temperature sensitive mutants of influenza virus
Identifieur interne : 002B70 ( Main/Exploration ); précédent : 002B69; suivant : 002B71Temperature sensitive mutants of influenza virus
Auteurs : Douglas D. Richman [États-Unis] ; Brian R. Murphy [États-Unis] ; Susan B. Spring [États-Unis] ; Marion T. Coleman [États-Unis] ; Robert M. Chanock [États-Unis]Source :
- Virology [ 0042-6822 ] ; 1975.
English descriptors
- Teeft :
- Assay, Attenuated, Attenuation, Chanock, Clone, Growth restriction, Hamster, Hemagglutinin, Higher temperature, Honl, Influenza, Influenza virus, Lesion, Lung homogenate, Mutant, Nasal, Nasal turbinates, Nasal turbinates lungs, Neuraminidase, Neuraminidase genes, Parent hong, Permissive temperature, Phenotype, Plaque, Plaque assay, Plaque formation, Rabies virus, Recombinant, Recombinant clone, Recombinant clones, Recombinant virus, Recombinant viruses, Restrictive temperature, Restrictive temperatures, Reversion, Revertant, Revertant virus, Richman, Shutoff, Shutoff recombinants, Shutoff temperature, Subset, Temperature sensitivity, Tissue culture, Turbinate, Vaccine, Viral, Viral growth, Virus, Wild type.
Abstract
Abstract: The temperature-sensitive (ts) mutant recombinant influenza A/Hong Kong/1968-ts-1[E] (H3N2) previously was shown to possess properties desirable for a live attenuated vaccine. We investigated the regularity of transfer of these desirable properties by recombination to a newly emerged wild-type influenza A (H3N2) variant, A/Udorn/307/1972. Examination of Udorn/72-ts-1[E] recombinant clones confirmed that the ts-1[E] donor contained two independently segregating ts lesions, each of which segregated independently of the hemagglutinin and neuraminidase genes. Transfer of both of these ts lesions to a new recombinant was associated with a specific shutoff temperature in vitro of 38°, a moderate restriction of growth in the respiratory tract of hamsters, and infrequent reversion to ts+ phenotype in vivo. The absence of one of the two ts lesions in a Udorn/72-ts-1[E] recombinant clone resulted in a higher in vitro shutoff temperature (39°), less growth restriction in hamsters, and less genetic stability with regard to reversion to the ts+ phenotype. These results supported the hypothesis that well-defined ts lesions can be utilized as specific markers of attenuation which can be transferred to new, potentially epidemic, antigenic variants of influenza A virus.
Url:
DOI: 10.1016/0042-6822(75)90227-5
Affiliations:
Links toward previous steps (curation, corpus...)
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- to stream Istex, to step Curation: 000473
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Le document en format XML
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We investigated the regularity of transfer of these desirable properties by recombination to a newly emerged wild-type influenza A (H3N2) variant, A/Udorn/307/1972. Examination of Udorn/72-ts-1[E] recombinant clones confirmed that the ts-1[E] donor contained two independently segregating ts lesions, each of which segregated independently of the hemagglutinin and neuraminidase genes. Transfer of both of these ts lesions to a new recombinant was associated with a specific shutoff temperature in vitro of 38°, a moderate restriction of growth in the respiratory tract of hamsters, and infrequent reversion to ts+ phenotype in vivo. The absence of one of the two ts lesions in a Udorn/72-ts-1[E] recombinant clone resulted in a higher in vitro shutoff temperature (39°), less growth restriction in hamsters, and less genetic stability with regard to reversion to the ts+ phenotype. These results supported the hypothesis that well-defined ts lesions can be utilized as specific markers of attenuation which can be transferred to new, potentially epidemic, antigenic variants of influenza A virus.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Géorgie (États-Unis)</li></region></list><tree><country name="États-Unis"><noRegion><name sortKey="Richman, Douglas D" sort="Richman, Douglas D" uniqKey="Richman D" first="Douglas D" last="Richman">Douglas D. Richman</name></noRegion><name sortKey="Chanock, Robert M" sort="Chanock, Robert M" uniqKey="Chanock R" first="Robert M" last="Chanock">Robert M. Chanock</name><name sortKey="Chanock, Robert M" sort="Chanock, Robert M" uniqKey="Chanock R" first="Robert M" last="Chanock">Robert M. Chanock</name><name sortKey="Coleman, Marion T" sort="Coleman, Marion T" uniqKey="Coleman M" first="Marion T" last="Coleman">Marion T. Coleman</name><name sortKey="Coleman, Marion T" sort="Coleman, Marion T" uniqKey="Coleman M" first="Marion T" last="Coleman">Marion T. Coleman</name><name sortKey="Murphy, Brian R" sort="Murphy, Brian R" uniqKey="Murphy B" first="Brian R" last="Murphy">Brian R. Murphy</name><name sortKey="Murphy, Brian R" sort="Murphy, Brian R" uniqKey="Murphy B" first="Brian R" last="Murphy">Brian R. Murphy</name><name sortKey="Richman, Douglas D" sort="Richman, Douglas D" uniqKey="Richman D" first="Douglas D" last="Richman">Douglas D. Richman</name><name sortKey="Spring, Susan B" sort="Spring, Susan B" uniqKey="Spring S" first="Susan B" last="Spring">Susan B. Spring</name><name sortKey="Spring, Susan B" sort="Spring, Susan B" uniqKey="Spring S" first="Susan B" last="Spring">Susan B. Spring</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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